(C) Ratio of depletion of Vex +, CD11b high splenocytes after 1 h FlaTox treatment over LFn-Fla alone treatment. (D and E) WT and RagA/B DKO 293T cells were transfected with plasmids encoding hGSDMD variants and normalized PI fluorescence (D) and cell-free supernatant LDH activity (E) were analyzed 20 h after transfection..