Echocardiography was performed using the Vevo 2,100 high-resolution ultrasound imaging system with a real-time 30 MHz linear array scanhead (MS400) at a frame rate of 235 fps, a focal length of 8 mm, and a 10 × 10-mm field of view (Visualsonics; Toronto, Canada). 8-12 week-old, male mice were anaesthetised with 2% isofluorane, and hair was ....