Xymed #180067), pLKB1 (1:1000, Cell Signaling Technology #C6783),pFOXO1Ser-256(1:1000,CellSignalingTechnology #9461). For CRTC2 (1:5000), CREB (1:5000), and pCREB (1:5000), rabbit polyclonal antibodies were raised against respective antigens (Salk Institute). For all Western blots, sec-ondary antisera were HRP-conjugated goat anti rabbit IgGs.